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KMID : 0357319920270040345
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Journal of the Korean Society for Microbiology
1992 Volume.27 No. 4 p.345 ~ p.358
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In Vitro Effect of Ciprofloxacin on Vibrio vulnificus
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류필열
신부안/이준행/정선식/안태휴
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Abstract
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In order to study the mechanism of the antimicrobial action of ciprofoxacin of Vibrio vulnificus, changes both in the function and in the morphology of the cells exposed in vitro to ciprofloxacin were studied in this investigation.
In vitro ciprofloxacin treatment of Vibrio vulnificus resulted in rapid decline of cell viability with concomitant increase in the trbidity of bacterial suspension. Light and electron microscopic studies of bacterial cells following ciprofloxacin
treatment were performed to explain these observations. The ciprofloxacin-ftreated cells continued to elongate with subsequent formation of filament; particularly, the cells exposed to sub-minimum inhibitory concentration of ciprofloxacin for 1
hour
became elongated to the length about 10 times those of the control. These elongated cells showed rather smooth cell wall-surface and abscence of septum formation in the division site when examined by electron microscope. By ciprofloxacin
treatment(5㎍/ml) for 1 hour, the cell membrane appeared to be detached from cytoplasm.
Furthermore ciprofloxacin incresed the suseptibility of bacterial cells to SDS-mediated lysis and the cell-surface hydrophobicity of the cells and resulted in the decrease of number of cells in postreplication period.
These findings suggest that the inhibition of DNA replication by ciprofloxachin brings on morphological changes and a cascade of physiological events terminating in a loss of membrane integrity, which leads ultimately to the lethal event in these
cells.
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